Wei, C. L., Yang, Y. B., Liu, W. C., Chen, W. Y., Liaw,
S. H.*, and Ysai, Y. C.* Mutational Shuffling of
Streptomyces clavuligerus Deacetoxycephalosporin C Synthase to
Improve Penicillin G Expansion. In submission.
Chen, S. C., Shen, C. Y., Yen, T. M., Yu, H. C., Chang, T. H.,
Lai, W. L., and Liaw, S. H.*
Evolution of vitamin B2 biosynthesis: eubacterial RibG and
fungal Rib2 deaminases. Acta Cryst. D In press
Huang, Y. L., Chung, T. W., Chen, C. H, Chang, C. M., Liao, C.
C., Tsay, Y. G., Shaw, G. C., Liaw,
S. H., Sun, C. M., and Lin, C. H.* Qualitative analysis
of the fluorophosphonate-based chemical probes using the serine
hydrolases from mouse liver and poly-3-hydroxybutyrate
depolymerase (PhaZ) from Bacillus thuringiensis Anal. Bioanal.
Chem. In press
Huang, T. H., Peng, H. J., Su, S. N., and Liaw,
S. H.* (2012) Various cross-reactivity of the grass
pollen group 4 allergens: crystallographic study of the Bermuda
grass isoallergen Cyn d 4. Acta Cryst. D68,
1303-1310
Chen, C. S., Chiou, C. T., Chen, G., Chen, S. C., Hu, C. Y.,
Chi, W. K., Chu, Y. D., Hwang, L. H., Chen, P. J., Liaw,
S. H.*, Chen, D. S.*, and Chern, J. W.* (2009)
Structure-based discovery of triphenylmethane derivatives as
inhibitors of hepatitis C virus helicase. J. Med. Chem.
52, 2716-2723
Chen, S. C., Lin, Y. H, Yu, H. C., and Liaw,
S. H.* (2009) Complex structure of Bacillus subtilis
RibG: the reduction mechanism during riboflavin biosynthesis. J.
Biol. Chem. 284, 1725-1731
Huang, C. H., Winkler, A., Chen, C. L., Lai, W. L., Tsai, Y.
C., Macheroux, P., and Liaw, S.
H.* (2008) Functional roles of the 6-S-cysteinyl,
8-N1-histidyl FAD in glucooligosaccharide oxidase from
Acremonium strictum. J. Biol. Chem. 283,
30990-30996
Chen, S. C., Chang, Y. C, Lin, C. H, Lin, C. H., and Liaw,
S. H.* (2006) Crystal structure of Bacillus subtilis
RibG: The ring structure of the deaminase-reductase in the
riboflavin biosynthesis. J. Biol. Chem. 281,
7605-7613
Huang, C. H., Lai, W. L., Lee, M. H., Chen, C. J., Vasella,
A., Tsai, Y. C., and Liaw, S. H.*
(2005) Crystal structure of glucooligosaccharide oxidase from
Acremonium strictum: A novel flavinylation of 6-S-cysteinyl,
8a-N1-histidyl FAD. J. Biol. Chem. 280,
38831-38838.
Lai, W. L., Lee, M. H., Liaw,
S. H.*, and Tsai, Y. C. (2005) Structural
characterization of glucooligosaccharide oxidase from Acremonium
strictum. Appl. Environ. Microbiol. 71, 8881-8887.
Wei, C. L.,Yang, Y. B., Deng, C. H., Liu, W. C., Hsu, J. S.,
Lin, Y. C., Liaw, S. H.*,
and Tsai, Y. C.* (2005) Directed evolution of Streptomyces
clavuligerus deacetoxycephalosporin c synthase for enhancement
of penicillin G expansion. Appl. Environ. Microbiol.
71, 8873-8870.
Tsai, M. L., Liaw, S. H.*,
and Chang, N. C.* (2004) The high-resolution crystal structure
of Ym1 suggests a lack of N-acetylglucosamine-binding affinity.
J. Struct. Biol. 148, 290-296.
Hsu, J. S., Yang, Y. B., Deng, C. H., Wei, C. L., Liaw,
S. H.*, and Tsai, Y. C.* (2004) Family shuffling of
expandase genes to enhance substrate specificty to penicillin G.
Appl. Environ. Microbiol. 70, 6257-6263.
Liaw, S. H.*, Chang, Y.
J., Lai, C. T., Chang, H. C., and Chang, G. G. (2004) Crystal
structure of the Bacillus subtilis guanine deaminase. The first
domain-swapped structure in the cytidine deaminase superfamily.
J. Biol. Chem. 279, 35479-35485.
Lai, W. L., Chou, L. Y., Ting, C. Y., Kirby, R., Tsai, Y. C.,
Wang, A. H. J., and Liaw, S. H.*
(2004) The functional role of the binuclear metal center in
D-aminoacylase. One-metal activation and second-metal
inhibition. J. Biol. Chem. 279, 13962-13967.
Ko, T.P., Lin, J. J., Hu, C. Y., Hsu, Y. H., Wang, A. H. J.,
and Liaw, S. H.* (2003)
Crystal structure of the yeast cytosine deaminase. Insights into
the enzyme mechanism and evolution. J. Biol. Chem.
278, 19111-19117.
Liaw, S. H., Chen, S. J.,
Ko, T. P., Hsu, C. S., Chen, C. J., Wang, A. H. J.*, and Tsai,
Y. C.* (2003) Crystal Structure of D-aminoacylase from
Alcaligenes faecalis DA1. A Novel subset of amidohydrolases and
insights into the enzyme mechanism. J. Biol. Chem.
278, 4957-4962.