• 專任副教授
  • 李曉暉
  • Hsiao-Hui Lee
  • (O)02-2826-7205/(Lab) 02-2826-7000 ext. 66257 or 66213
  • hhl@nycu.edu.tw
  • 圖書資訊暨研究大樓9樓R923、R926
  • 專業領域
  • 細胞與分子生物學 (Cellular and molecular Biology)、應力生物學 (mechanobiology)、腎臟基礎研究 (renal basic research)
  • 研究方向
  • 細胞會因應胞外環境的各種化學與物理的訊號,透過訊息傳遞調節細胞的各種活性。我們實驗室主要在探討調控細胞骨架的ROCK分子,並發現ROCK的調控對於足細胞 (podocyte) 的附著與細胞骨架結構的維持扮演關鍵角色。足細胞是腎臟進行血液過濾最關鍵的細胞,他們在腎絲球中藉由特殊的足突形成如過濾膜般的結構以防止蛋白尿的發生。由於足細胞是完全分化無法再生的細胞,因此足細胞的受損與剝離是慢性腎病惡化至末期而需洗腎的最重要關鍵之一。許多研究指出在各種原發性腎絲球腎炎或繼發性如糖尿病腎病變等的病程中,血液過濾所產成的機械性應力扮演重要角色,然而目前這方面的研究卻十分有限。我們實驗室建立了模擬細胞面對過濾的模組,以誘發分化的足細胞探討過濾應力對足細胞的細胞骨架與基因表現的影響。另一方面,我們也積極建立各種足細胞受損的細胞實驗模組,藉以了解足細胞受損的機制以及對藥物的反應,希望在未來能藉此能提供新的想法或策略來減少腎絲球病變的發生與惡化。
    My lab focuses on the cell adhesion and cytoskeletal structure of podocytes. Podocytes are terminal differentiated glomerular epithelial cells. They play a crucial role for blood filtration in kidney. We are establishing various of podocyte injury cell models (genetic, in-vitro filtration, drug-induced, high-glucose, etc.) as platforms to explore the mechanisms of podocyte dysfunction and to develop new therapeutic strategy.

  • 教授科目
  • 生物學
    生理學 (英文授課)
    分子細胞生物概論 (英文授課)
  • 經歷
  • 2015 Feb.-present
  • 2013 Aug.-2015 Jan.
  • 2009 Aug.-2013 Jul.
  • 2011 Apr., Jul.-Sep., 2012 Jan.-Feb.
  • 2006 Feb.-2009 Jul.
  • 學歷
  • 2006 Jan.
  • 1998
  • 1996


  • Tseng CC, Zheng RH, Lin TW, Chou CC, Shih YC, Liang SW, Lee HH*. α-Actinin-4 recruits Shp2 into focal adhesions to potentiate ROCK2 activation in podocytes. Life Sci Alliance. 2022 Sep 12;5(11):e202201557. doi: 10.26508/lsa.202201557. (*corresponding author)
  • Chang Chien CY, Chou SH, Lee HH*. Integrin molecular tension required for focal adhesion maturation and YAP nuclear translocation. Biochem Biophys Rep. 2022 May31;31:101287. doi: 10.1016/j.bbrep.2022.101287. (*corresponding author)
  • Lisowska J, Rödel CJ, Manet S, Miroshnikova YA, Boyault C, Planus E, De Mets R, Lee HH, Destaing O, Mertani H, Boulday G, Tournier-Lasserve E, Balland M, Abdelilah-Seyfried S, Albiges-Rizo C, Faurobert E. L. The CCM1-CCM2 complex controls complementary functions of ROCK1 and ROCK2 that are required for endothelial integrity. J Cell Sci. 2018 Aug 13;131(15). pii: jcs216093. doi: 10.1242/jcs.216093.
  • Jheng GW, Hur SS, Chang CM, Wu CC, Cheng JS, Lee HH, Chung BC, Wang YK, Lin KH, Del Álamo JC, Chien S, Tsai JW. Lis1 dysfunction leads to traction force reduction and cytoskeletal disorganization during cell migration. Biochem Biophys Res Commun. 2018 Mar 11;497(3):869-875. doi: 10.1016/j.bbrc.2018.02.151.
  • Chang CH, Lee HH*, Lee CH*. Substrate properties modulate cell membrane roughness by way of actin filaments. Sci Rep. 2017 Aug 22;7(1):9068. doi: 10.1038/s41598-017-09618-y (*corresponding author)
  • Wu TH, Kuo YY, Lee HH, Kuo JC, Ou MH, Chang ZF*. Epigenetic repression of ribosomal RNA transcription by ROCK-dependent aberrant cytoskeletal organization. Sci Rep. 2016 Jun 28;6:28685. doi: 10.1038/srep28685.
  • Tien SC, Lee HH, Yang YC, Lin MH, Chen YJ, Chang ZF*. Inhibition of HDAC6 reverses oncogenic Shp2-induced epithelial lumenogenesis defect independent of Cdc42. Nature Communications. 2016 Jan 19;7:10420. doi: 10.1038/ncomms10420.
  • Hsu CY, Chang ZF, and Lee HH*. Immunohistochemical evaluation of ROCK activation in invasive breast cancer. BMC Cancer. 2015 Dec 1;15(1):943. doi: 10.1186/s12885-015-1948-8. (*corresponding author)
  • Liu NY, Lee HH, Chang ZF, Tsay YG*. Examination of segmental average mass spectra from liquid chromatography-tandem mass spectrometric (LC-MS/MS) data enables screening of multiple types of protein modifications. Anal Chim Acta. 2015 Sep 10;892: 115-22. doi: 10.1016/j.aca.2015.07.032.
  • Wang HL, Yang CH, Lee HH, Kuo JC, Hur SS, Chien S, Lee KS, Hung SH, Chang ZF*. Dexamethasone-induced SGK1 increases Sec5/GEF-H1 interaction essential for adhesion-mediated tension. J Cell Sci. 2015 Oct 15;128(20):3757-68. doi: 10.1242/jcs.169961.
  • Tsai IJ, Chou CH, Yang YH, Lin WC, Lin YH, Chow LP, Lee HH, Kao PG, Liau WT, Jou TS*, Tsau YK*. Inhibition of Rho-associated kinase relieves C5a induced proteinuria in murine nephrotic syndrome. Cell Mol Life Sci. 2015 Aug;72(16):3157-71. doi: 10.1007/s00018-015-1888-0.
  • Kuo YC, Chang TH, Hsu WT, Zhou J, Lee HH, Ho HC, Chien S, and Lee KS*. Oscillatory shear stress mediates directional re-organization of actin cytoskeleton and alters differentiation propensity of mesenchymal stem cells. Stem Cells. 2015 Feb;33(2):429-42. doi: 10.1002/stem.1860.
  • Chuang HH, Liang SW, Chang ZF*, and Lee HH*. Ser1333 phosphorylation indicates ROCKI activation. J Biomed Sci. 2013 Oct 29;20:83. doi: 10.1186/1423-0127-20-83. (*corresponding author)
  • Lee HH*, Lee HC, Chou CC, Hur SS, Osterday K, del Álamo JC, Lasheras JC, and Chien S*. Shp2 plays a crucial role in cell structural orientation and force polarity in response to matrix rigidity. Proc Natl Acad Sci U S A. 2013 Feb 19;110(8):2840-5. doi: 10.1073/pnas.1222164110. (* corresponding author)
  • Pan YR, Cho KH, Lee HH, Chang ZF, and Chen HC. Protein tyrosine phosphatase SHP2 suppresses podosome rosette formation in Src-transformed fibroblasts. J Cell Sci. 2013 Jan 15;126(Pt 2):657-66. doi: 10.1242/jcs.116624.
  • Chuang HH , Yang CH, Tsay YG, Hsu CY, Tseng LM, Chang ZF*, Lee HH*. ROCKII serine 1366 phosphorylation reflects the activation status. Biochem J. 2012 Apr 1;443(1):145-51. doi: 10.1042/BJ20111839. (*corresponding authors)
  • Lee HH, Tien SC, Jou TS, Chang YC, Jhong JG, and Chang ZF. c-Src-dependent phosphorylation of ROCK participates in regulation of focal adhesion dynamics. J Cell Sci. 2010 Oct 1;123(Pt 19):3368-77. doi: 10.1242/jcs.071555.
  • Lee HH and Chang ZF. Regulation of RhoA-dependent ROCKII activation by Shp2. J Cell Biol. 2008 Jun 16;181(6):999-1012. doi: 10.1083/jcb.200710187. (Faculty of 1000 in Biology, Recommended)
  • Chang YC, Lee HH, Chen YJ, Bokoch G and Chang, ZF. Contribution of guanine exchange factor H1 in phorbol ester-induced apoptosis. Cell Death Differ. 2006 Dec;13(12):2023-32.
  • Chang ZF and Lee HH. RhoA signaling in phorbol ester-induced apoptosis. J Biomed Sci. 2006 Mar;13(2):173-80. (Review)
  • Lee HH, Chien CL, Liao HK, Chen YJ, and Chang ZF. Nuclear efflux of heterogeneous nuclear ribonucleoprotein C1/C2 in apoptotic cells: a novel nuclear export dependent on Rho-associated kinase activation. J Cell Sci. 2004 Nov 1;117(Pt 23):5579-89.
  • Lai LP, Lin JL, Lin CS, Yeh HM, Tsay YG, Lee CF, Lee HH, Chang ZF, Hwang JJ, Su MJ, Tseng YZ, Huang SK. Functional genomic study on atrial fibrillation using cDNA microarray and two-dimensional protein electrophoresis techniques and identification of the myosin regulatory light chain isoform reprogramming in atrial fibrillation. J Cardiovasc. Electrophysiol. 2004 Feb;15(2):214-23.
  • Lin CM, Chen CT, Lee HH, Lin JK. Prevention of cellular ROS damage by isovitexin and related flavonoids. Planta Med. 2002 Apr;68(4):365-7.


  • 專利名稱:Reagents for probing ROCKs activation.
    發明人:Zee-Fen CHANG, Hsiao-Hui Lee
    公告號 (TW):I460274 (Taiwan patent approved at 2014)
    公開號 (US):US9017952 B2 (US patent approved at 2015)