教師簡介

  • 專任副教授
  • 李曉暉
  • Hsiao-Hui Lee
  • (O)02-2826-7205/(Lab) 02-2826-7000 ext. 66257 or 66213
  • hhl@nycu.edu.tw
  • 圖書資訊暨研究大樓9樓R923、R926
  • 專業領域
  • • 細胞與分子生物學(Cellular and molecular Biology)、細胞骨架(Cytoskeleton)、應力生物學(mechanobiology)、腎臟學基礎研究(Basic Research in Nephrology)
  • 研究方向
  • 研究核心:細胞骨架與力學訊號傳導
    我們實驗室的研究關注於細胞骨架與細胞力學的調控機制。細胞會接收來自外界的生化或物理訊號,並將這些訊號轉化為細胞內的指令,以調控細胞的形狀、附著與功能。我們發現細胞內Shp2分子能調控ROCK2,在細胞與基質接觸的焦點黏附處,幫助細胞感知外在的物理環境;而α-actinin-4分子則參與細胞骨架的穩定與黏附能力,尤其在腎臟的足細胞中扮演重要角色。
    Core Research: Cytoskeletal Regulation and Mechanotransduction
    Our lab focuses on how cells sense and respond to biochemical and physical cues from their environment. These signals are transduced into intracellular pathways that regulate cytoskeletal organization and cell mechanics. We have previously demonstrated that Shp2 regulates ROCK2 at focal adhesions, enhancing cell adhesion and cytoskeletal integrity through α-actinin-4-particularly in podocytes.

    足細胞與腎臟疾病模型研究
    足細胞是腎臟進行血液過濾最關鍵的細胞,具有特殊的足突結構形成過濾膜以防止蛋白質流失,因此,足細胞的受損往往會導致蛋白尿的發生,進而發展成慢性腎病。且因足細胞高度分化且不可再生的特性,其長期損傷或脫落,將導致慢性腎病的惡化,甚至進展至需洗腎的末期腎病。我們實驗室利用可誘導分化的足細胞株,並研發模擬過濾應力的體外實驗系統,嘗試探討其在受力狀態下的細胞骨架變化與基因表現改變;同時開發各種足細胞損傷模型,以了解不同腎毒性刺激下的足細胞損傷機制與藥物反應。希望透過這些研究,未來能提出新的策略,預防或延緩腎絲球病變的發生與惡化。
    Podocytes and Kidney Disease Modeling
    Podocytes are specialized glomerular epithelial cells forming the filtration barrier in the kidney. Their damage or detachment is a critical event in the progression of chronic kidney diseases. Our lab has developed an in vitro filtration-stress model to study cytoskeletal remodeling and transcriptional responses in differentiated podocytes. We also investigate various injury models to understand podocyte dysfunction and therapeutic responses.

  • 教授科目
  • 生物學
    生命科學總論
    細胞生物學
    生命科學實驗
    進階細胞生物學
    細胞骨架力學
    藥物化學概論
    健康與疾病通論
    跨領域科學在細胞移動研究之應用
    生理學 (英文授課)
    分子細胞生物概論 (英文授課)
  • 經歷
  • 2015 Feb.-present
    國立陽明交通大學
    生命科學系暨基因體科學研究所
    副教授
  • 2013 Aug.-2015 Jan.
    國立陽明大學
    生命科學系暨基因體科學研究所
    助理教授
  • 2009 Aug.-2013 Jul.
    國立陽明大學
    生命科學系暨基因體科學研究所
    專案助理教授
  • 2011 Apr., Jul.-Sep., 2012 Jan.-Feb.
    美國加州大學聖地牙哥分校
    醫學工程研究所
    訪問學者
  • 2006 Feb.-2009 Jul.
    國立臺灣大學
    生物化學暨分子生物學研究所
    博士後研究
  • 學歷
  • 2006 Jan.
    國立臺灣大學
    生物化學暨分子生物學研究所
    博士
  • 1998
    臺北醫學院
    醫學科學所
    碩士
  • 1996
    臺北醫學院
    藥學系
    學士

代表著作

    期刊論文
  • Huang XY, Lu YH, Lee HH*. Targeting Heparanase Attenuates Podocyte Injury Induced by Puromycin Aminonucleoside. J Cell Physiol. 2025 Jun;240(6):e70053. (*corresponding author)
  • Chen YQ, Lee HC,Lee HH*. Shp2 contributes to the regulation of nuclear shape and cellular viscoelasticity in response to substrate spatial cues. Biochem Biophys Res Commun. 2024 Aug 20; 721:1501443.(*corresponding author)
  • Tseng CC, Zheng RH, Lin TW, Chou CC, Shih YC, Liang SW, Lee HH*. α-Actinin-4 recruits Shp2 into focal adhesions to potentiate ROCK2 activation in podocytes. Life Sci Alliance. 2022 Sep 12;5(11):e202201557. doi: 10.26508/lsa.202201557. (*corresponding author)
  • Chang Chien CY, Chou SH, Lee HH*. Integrin molecular tension required for focal adhesion maturation and YAP nuclear translocation. Biochem Biophys Rep. 2022 May31;31:101287. doi: 10.1016/j.bbrep.2022.101287. (*corresponding author)
  • Lisowska J, Rödel CJ, Manet S, Miroshnikova YA, Boyault C, Planus E, De Mets R, Lee HH, Destaing O, Mertani H, Boulday G, Tournier-Lasserve E, Balland M, Abdelilah-Seyfried S, Albiges-Rizo C, Faurobert E. L. The CCM1-CCM2 complex controls complementary functions of ROCK1 and ROCK2 that are required for endothelial integrity. J Cell Sci. 2018 Aug 13;131(15). pii: jcs216093. doi: 10.1242/jcs.216093.
  • Jheng GW, Hur SS, Chang CM, Wu CC, Cheng JS, Lee HH, Chung BC, Wang YK, Lin KH, Del Álamo JC, Chien S, Tsai JW. Lis1 dysfunction leads to traction force reduction and cytoskeletal disorganization during cell migration. Biochem Biophys Res Commun. 2018 Mar 11;497(3):869-875. doi: 10.1016/j.bbrc.2018.02.151.
  • Chang CH, Lee HH*, Lee CH*. Substrate properties modulate cell membrane roughness by way of actin filaments. Sci Rep. 2017 Aug 22;7(1):9068. doi: 10.1038/s41598-017-09618-y (*corresponding author)
  • Wu TH, Kuo YY, Lee HH, Kuo JC, Ou MH, Chang ZF*. Epigenetic repression of ribosomal RNA transcription by ROCK-dependent aberrant cytoskeletal organization. Sci Rep. 2016 Jun 28;6:28685. doi: 10.1038/srep28685.
  • Tien SC, Lee HH, Yang YC, Lin MH, Chen YJ, Chang ZF*. Inhibition of HDAC6 reverses oncogenic Shp2-induced epithelial lumenogenesis defect independent of Cdc42. Nature Communications. 2016 Jan 19;7:10420. doi: 10.1038/ncomms10420.
  • Hsu CY, Chang ZF, and Lee HH*. Immunohistochemical evaluation of ROCK activation in invasive breast cancer. BMC Cancer. 2015 Dec 1;15(1):943. doi: 10.1186/s12885-015-1948-8. (*corresponding author)
  • Liu NY, Lee HH, Chang ZF, Tsay YG*. Examination of segmental average mass spectra from liquid chromatography-tandem mass spectrometric (LC-MS/MS) data enables screening of multiple types of protein modifications. Anal Chim Acta. 2015 Sep 10;892: 115-22. doi: 10.1016/j.aca.2015.07.032.
  • Wang HL, Yang CH, Lee HH, Kuo JC, Hur SS, Chien S, Lee KS, Hung SH, Chang ZF*. Dexamethasone-induced SGK1 increases Sec5/GEF-H1 interaction essential for adhesion-mediated tension. J Cell Sci. 2015 Oct 15;128(20):3757-68. doi: 10.1242/jcs.169961.
  • Tsai IJ, Chou CH, Yang YH, Lin WC, Lin YH, Chow LP, Lee HH, Kao PG, Liau WT, Jou TS*, Tsau YK*. Inhibition of Rho-associated kinase relieves C5a induced proteinuria in murine nephrotic syndrome. Cell Mol Life Sci. 2015 Aug;72(16):3157-71. doi: 10.1007/s00018-015-1888-0.
  • Kuo YC, Chang TH, Hsu WT, Zhou J, Lee HH, Ho HC, Chien S, and Lee KS*. Oscillatory shear stress mediates directional re-organization of actin cytoskeleton and alters differentiation propensity of mesenchymal stem cells. Stem Cells. 2015 Feb;33(2):429-42. doi: 10.1002/stem.1860.
  • Chuang HH, Liang SW, Chang ZF*, and Lee HH*. Ser1333 phosphorylation indicates ROCKI activation. J Biomed Sci. 2013 Oct 29;20:83. doi: 10.1186/1423-0127-20-83. (*corresponding author)
  • Lee HH*, Lee HC, Chou CC, Hur SS, Osterday K, del Álamo JC, Lasheras JC, and Chien S*. Shp2 plays a crucial role in cell structural orientation and force polarity in response to matrix rigidity. Proc Natl Acad Sci U S A. 2013 Feb 19;110(8):2840-5. doi: 10.1073/pnas.1222164110. (* corresponding author)
  • Pan YR, Cho KH, Lee HH, Chang ZF, and Chen HC. Protein tyrosine phosphatase SHP2 suppresses podosome rosette formation in Src-transformed fibroblasts. J Cell Sci. 2013 Jan 15;126(Pt 2):657-66. doi: 10.1242/jcs.116624.
  • Chuang HH , Yang CH, Tsay YG, Hsu CY, Tseng LM, Chang ZF*, Lee HH*. ROCKII serine 1366 phosphorylation reflects the activation status. Biochem J. 2012 Apr 1;443(1):145-51. doi: 10.1042/BJ20111839. (*corresponding authors)
  • Lee HH, Tien SC, Jou TS, Chang YC, Jhong JG, and Chang ZF. c-Src-dependent phosphorylation of ROCK participates in regulation of focal adhesion dynamics. J Cell Sci. 2010 Oct 1;123(Pt 19):3368-77. doi: 10.1242/jcs.071555.
  • Lee HH and Chang ZF. Regulation of RhoA-dependent ROCKII activation by Shp2. J Cell Biol. 2008 Jun 16;181(6):999-1012. doi: 10.1083/jcb.200710187. (Faculty of 1000 in Biology, Recommended)
  • Chang YC, Lee HH, Chen YJ, Bokoch G and Chang, ZF. Contribution of guanine exchange factor H1 in phorbol ester-induced apoptosis. Cell Death Differ. 2006 Dec;13(12):2023-32.
  • Chang ZF and Lee HH. RhoA signaling in phorbol ester-induced apoptosis. J Biomed Sci. 2006 Mar;13(2):173-80. (Review)
  • Lee HH, Chien CL, Liao HK, Chen YJ, and Chang ZF. Nuclear efflux of heterogeneous nuclear ribonucleoprotein C1/C2 in apoptotic cells: a novel nuclear export dependent on Rho-associated kinase activation. J Cell Sci. 2004 Nov 1;117(Pt 23):5579-89.
  • Lai LP, Lin JL, Lin CS, Yeh HM, Tsay YG, Lee CF, Lee HH, Chang ZF, Hwang JJ, Su MJ, Tseng YZ, Huang SK. Functional genomic study on atrial fibrillation using cDNA microarray and two-dimensional protein electrophoresis techniques and identification of the myosin regulatory light chain isoform reprogramming in atrial fibrillation. J Cardiovasc. Electrophysiol. 2004 Feb;15(2):214-23.
  • Lin CM, Chen CT, Lee HH, Lin JK. Prevention of cellular ROS damage by isovitexin and related flavonoids. Planta Med. 2002 Apr;68(4):365-7.

    專利與技轉

  • 專利名稱:Reagents for probing ROCKs activation.
    發明人:Zee-Fen CHANG, Hsiao-Hui Lee
    公告號 (TW):I460274 (Taiwan patent approved at 2014)
    公開號 (US):US9017952 B2 (US patent approved at 2015)
    技轉:億康生物科技有限公司