• 專任副教授
  • 張欣暘
  • Hsin-Yang Chang
  • 02-2826-7168/lab: ext. 65789 or 65771 or 65726
  • hychang5@nycu.edu.tw
  • 傳統醫學大樓甲棟1樓R108
  • 專業領域
  • 結構生物學 (Structural biology)、X-Ray蛋白晶體學(X-ray crystallography)、小角度散射 (SAXS: Small-angle X-ray scattering)、酵素動力學 (Enzyme kinetics)
  • 研究方向
  • (1)實驗室研發如何利用酵素生合成天然抗生素如Vitroprocines。我們目前發現其生合成反應機制需要兩個特別的酵素VsAOS-2和VsOR 來完成。除了生合成途徑,我們也專注於此類酵素在分子層次及三級結構之功能分析。此類抗生素可對抗多重抗藥性 Acinetobacter baumannii (鮑氏不動桿菌) 等在醫院常被感染之致病菌,如:肺炎、腦膜炎、皮膚組織感染及泌尿道感染。這項研究有助於新型抗生素研發。

  • (2)實驗室研究細胞膜蛋白如何進入生物膜的生理機制。大多數真核細胞膜蛋白質首先被插入內質網, 此過程稱為共同轉譯途徑 (co-translational pathway)。然而,有一類在生理功能上比較特別的膜蛋白,屬於尾端固定(tail-anchored, TA)膜蛋白家族,而是經由另一個稱為後轉譯途徑 (post-translational pathway) 的特殊新途徑完成。此途徑只有在真核細胞才有。錯誤的TA運送會導致癌症、糖尿病和心臟病等相關人類疾病。我們以真核物種為研究對象,探討TA 膜蛋白運如何被ArsAs (或Get3) 運輸蛋白送至各胞器外膜。這項研究對於TA 膜蛋白所引發的致病分子機制將有更進一步了解。

  • 教授科目
  • 生物化學
  • 經歷
  • Aug. 2020-present
  • Aug. 2019-Jul. 2020
  • Aug. 2015- Jul. 2019
  • 學歷
  • 2010 美國伊利諾大學香檳分校
    (University of Illinois at Urbana-Champaign)
    生物化學所 博士



  • CC Liaw, LH Lo, TH Cheng, YT Chan, YR Huang, AHJ Wang, HY Chang*(2022) Biosynthesis of Vitroprocines by Identified α-oxoamine Synthase and Oxidoreductase from Vibrio sp. QWI-06, ACS Organic Letters, https://doi.org/10.1021/acs.orglett.2c01178
  • HY Chang*, LH Lo, YH Lan, MX Hong, YT Chan, TP Ko, YR Huang, TH Cheng, CC Liaw (2021) Structural insights into the substrate selectivity of α-oxoamine synthases from marine Vibrio sp. QWI-06, Colloids Surf. B Biointerfaces, doi:https://doi.org/10.1016/j.colsurfb.2021.112224
  • HY Chang*, TH Cheng, AHJ Wang (2020) Structure, Catalysis and Inhibition Mechanism of Prenyltransferase (Critical Review). IUBMB Life, https://doi.org/10.1002/iub.2418
  • HC Lin, WH Li, CC Chen, TH Cheng, YH Lan, MD Huang, WM Chen, JS Chang, HY Chang*(2020) Diverse enzymes with industrial applications in four thraustochytrid genera. Front. Microbiol., https://doi.org/10.3389/fmicb.2020.573907.
  • CY Chiang, CC Chou, HY Chang, MF Hsu, PJ Pao, MH Chiang, AHJ Wang (2020) Biochemical and molecular dynamics studies of archaeal polyisoprenyl pyrophosphate phosphatase from Saccharolobus solfataricus. Enzyme Microb. Technol. doi.org/10.1016/ j.enzmictec.2020.109585
  • HL Yeh, TH Lin, CC Chen, TX Cheng, HY Chang, TM Lee (2019) Monodehydroascorbate reductase plays a role in the tolerance of Chlamydomonas reinhardtii to photooxidative stress. Plant Cell Physiol. 60: 2167–2179.
  • TW Lin, CC Chen, SM Wu, YC Chang, YC Li, YW Su, CD Hsiao, HY Chang*. (2019) Structural analysis of chloroplast tail-anchored membrane protein recognition by ArsA1, The Plant Journal, 99:128-143.
  • HY Chang*, TP Ko, YC Chang, KF Huang, CY Lin, HY Chou, CY Chiang, HJ Tsai. (2019) Crystal structure of the blue fluorescent protein with a Leu-Leu-Gly tri-peptide chromophore derived from the purple chromoprotein of Stichodactyla haddoni, Int. J. Biol. Macromol. 130: 675-684.
  • HY Chang*, ST Lin, TP Ko, SM Wu, TH Lin, YC Chang, KF Huang, TM Lee. (2017) Enzymatic characterization and crystal structure analysis of Chlamydomonas reinhardtii dehydroascorbate reductase and their implications for oxidative stress, Plant Physiol. Biochem. 120: 144-155.
  • M Maestre-Reyna, SM Wu, YC Chang, CC Chen, A Maestre-Reyna, AHJ Wang, HY Chang*. (2017) In search of tail-anchored protein machinery in plants: reevaluating the role of arsenite transporters, Scientific Report 7:46022 | DOI: 10.1038/srep46022.
  • HY Chang, CC Chou, ML Wu, AHJ Wang. (2017) Expression, purification and enzymatic characterization of undecaprenyl pyrophosphate phosphatase from Vibrio vulnificus, Protein Expr. Purif. 133: 121-131.
  • C von Ballmoos, I Smirnova, F Poiana, N Gonska, HY Chang, RB Gennis, P Brzezinski, P Ädelroth. (2017) Dynamics of the KB proton pathway in cytochrome ba3 from Thermus thermophiles, Isr. J. Chem. 57: 1-14. DOI: 10.1002/ijch.201600136.
  • ST Lin, CW Chiou, YL Chu, YH, YF Tseng, YC Chen, HJ Chen, HY Chang, TM Lee. (2016) Enhanced ascorbate regeneration via dehydroascorbate reductase confers tolerance to photo-oxidative stress in Chlamydomonas reinhardtii. Plant Cell Physiol. 10: 2104-2121.
  • A Mateja, M Paduch, HY Chang, A Szydlowska, AA Kossiakoff, RS Hegde, RJ Keenan. (2015) Structure of the Get3 targeting factor in complex with its membrane protein cargo. Science. 347: 1152-1155.
  • HY Chang, CC Chou, MF Hsu, AHJ Wang. (2014) Proposed carrier lipid-binding site of undecaprenyl pyrophosphate phosphatase from Escherichia coli. J. Biol. Chem. 289: 18719-18735.
  • I Smirnova, HY Chang, C von Ballmoos, P Ädelroth, RB Gennis, P Brzezinski. (2013) Single mutations that redirect internal proton transfer in the ba3 oxidase from Thermus thermophilus. Biochemistry 52: 7022-7030.
  • HY Chang, SK Choi, AS Vakkasoglu, Y Chen, J Hemp, JA Fee, RB Gennis. (2012) Exploring the proton pump and exit pathway for pumped protons in cytochrome ba3 from Thermus thermophilus. Proc Natl Acad Sci U S A 109: 5259-5264.
  • HY Chang, Y Ahn, LA Pace, MT Lin, YH Lin, RB Gennis. (2010) The diheme cytochrome c4 from Vibrio cholerae is a natural electron donor to the cbb3-type cytochrome c oxidase. Biochemistry 49: 7494-7503.
  • I Smirnova, J Reimann, C von Ballmoos, HY Chang, RB Gennis, JA Fee, P Brzezinski, P Ädelroth. (2010) Functional role of Thr-312 and Thr-315 in the proton-transfer pathway in ba3 cytochrome c oxidase from Thermus thermophilus. Biochemistry 49: 7033-7049.
  • HY Chang, J Hemp, Y Chen, JA Fee, RB Gennis. (2009) The cytochrome ba3 oxygen reductase from Thermus thermophilus uses a single input channel for proton delivery to the active site and for proton pumping. Proc Natl Acad Sci U S A 106: 16169-16173.
  • TH Kuo, SC Chuang, SY Chang, PH Liang. (2006) Ligand specificities and structural requirements of two Tachypleus plasma lectins for bacterial trapping. Biochem. J. 393: 757-766.
  • APC Chen, SY Chang, YC Lin, YS Sun, CT Chen, AHJ Wang, PH Liang. (2005) Substrate and product specificities for cis-type undecaprenyl pyrophosphate synthase. Biochem. J. 386: 169-176.
  • SY Chang, TP Ko, APC Chen, AHJ Wang, PH Liang. (2004) Substrate binding mode and reaction mechanism of undecaprenyl pyrophosphate synthase deduced from crystallographic studies. Protein Sci. 13: 971-978.
  • SY Chang, TP Ko, PH Liang, AHJ Wang. (2003) Catalytic mechanism revealed by the crystal structure of undecaprenyl pyrophosphate synthase in complex with sulfate, magnesium, and Triton. J. Biol. Chem. 278: 29298-29307.
  • SY Chang, YK Chen, AHJ Wang, PH Liang. (2003) Identification of the active conformation and the importance of length of the flexible loop 72-83 in regulating the conformational change of undecaprenyl pyrophosphate synthase. Biochemistry. 42: 14452-14459.
  • CE Atreya, EF Johnson, J Williamson, SY Chang, PH Liang, KS Anderson. (2003). Probing electrostatic channeling in protozoal bifunctional thymidylate synthase-dihydrofolate reductase using site-directed mutagenesis. J. Biol. Chem. 278: 28901- 28911.
  • SY Chang, PC Tsai, CS Tseng, PH Liang. (2001) Refolding and characterization of a yeast dehydrodolichyl diphosphate synthase overexpressed in Escherichia coli. Prot. Exp. Puri. 23: 432-43.