其他師資
專任師資
兼任師資
合聘師資
退休師資
教師簡介
- 專任教授
- 羅清維
- Ching-Wei Luo
- 02-2826-7185／lab: ext. 65779
- luocw@nycu.edu.tw
- 傳統醫學大樓甲棟1樓 R111
- 實驗室網頁
- http://cwluo.lab.nycu.edu.tw/
- 專業領域
- 間質幹細胞分化機轉、生殖系統生理與癌症、激素或細胞因子藥物開發、訊息傳遞等分子內分泌學
- 研究方向
-
激素或細胞因子是主導各種生理反應的重要分子，一直以來都是生醫研究和藥物開發的重點標的。本實驗室的研究重點有二：(1)致力於以分子內分泌學的技術來探討蛋白質類的激素和細胞因子如何透過對應受體的整合訊息去影響生理反應、疾病 (如癌症等) 或幹細胞分化的相關進程；(2) 透過基因體學分析和遺傳工程重組技術，開發或設計新型的蛋白質激素和細胞因子，以評估其機轉、生物活性和成為蛋白質候選藥物的潛力。以下簡要說明幾個研究主題:
Project 1. TGF-beta/BMP 訊息對於幹細胞分化與細胞功能的影響。
本實驗室利用解析細胞訊息、佐以原代細胞培養、小鼠生理模式、疾病相關體學分析等，探討TGF-beta/BMP家族的訊息對間質幹細胞 (mesenchymal stem cell)、生殖幹細胞與體細胞的功能影響。希望未來能在骨癒合、骨質疏鬆或不孕症等領域有其應用價值。
Project 2. 卵巢生理調控與生殖系統相關癌症的致病機轉
卵巢除了是掌控生理和生殖的重要器官之外，其衍生癌症也是婦科癌症中致死率最高且復發率最高的癌種。我們的研究策略是利用基因體分析，找出在卵巢週期或癌症病程中具有明顯變化的蛋白激素或受體基因，進而設計細胞模式、原代細胞實驗以操控細胞訊息與基因表現、再佐以小鼠活體驗証等，藉此了解特定的基因功能和卵巢癌致病機轉。
Project 3. 開發新型的蛋白質激素或候選藥物
蛋白質型藥物具有比一般化學藥物更高的受體結合專一度，因此具有極佳的生理相容性和極低副作用。本實驗室的策略是利用重組和突變等技術改良具有蛋白質型藥物潛力的激素或細胞因子，試圖克服其折疊效率低和生理半衰期短的缺點，並佐以細胞訊息分析和小鼠實驗評估其作為候選藥物的潛力。目前的研究重點在胱胺酸結狀蛋白 (cystine-knot protein) 系列，例如長效型血管內皮細胞生長因子已進入專利審查階段。
- 教授科目
- 生物學/生物學實驗
生科總論
分子生物學
論文閱讀與分析方法
生命科學技術
生命科學教學
- 經歷
- Feb, 2017-present
國立陽明交通大學
生命科學系暨基因體科學研究所
教授 - Aug, 2010-Jan, 2017
國立陽明大學
生命科學系暨基因體科學研究所
副教授 - Aug, 2006-Jul, 2010
國立陽明大學
生命科學系暨基因體科學研究所
助理教授 - Nov, 2005-Jul, 2006
國立陽明大學生命科學系
助理教授 - May 2002-Oct, 2005
Stanford Univ. Medical Center
博士後研究員 - Jul, 2001-Apr, 2002
中央研究院生物化學所
博士後研究員 - Jan, 2001-Jun, 2001
國立臺灣大學生化科學所
博士後研究員 - 學歷
- Sep, 1996-Dec, 2000
國立臺灣大學
生化科學研究所 博士 - Sep, 1992-Jun, 1994
國立臺灣大學
生化科學研究所 碩士 - Sep, 1988-Jun, 1992
國立臺灣大學
農業化學系 學士
代表著作
- Wang YW, Wu CH, Lin TY,Expression profiling of ovarian BMP antagonists reveals the potential interaction between TWSG1 and the chordin subfamily in the ovary. Molecular and Cellular Endocrinology.(PubMed)(Share link)
- Wu FJ, Wang YW, Human bone morphogenetic protein 8A promotes expansion and prevents apoptosis of cumulus cells in vitro. Molecular and Cellular Endocrinology.(PubMed)(Share link)
- Wu FJ, Wang YW, Human BMP8A suppresses luteinization of rat granulosa cells via the SMAD1/5/8 pathway. Reproduction. Mar;159(3):315-324. (PubMed)
- Kuo CL, Jiang ZY, Wang YW, Lin TY, Huang WL, Wu FJ, . (2019) In vivo selection reveals autophagy promotes adaptation of metastatic ovarian cancer cells to abdominal microenvironment. Cancer Science. Aug 5. doi: 10.1111/cas.14162. (PubMed)
- Wu FJ, . (2017) Discovery of spermatogenic activators: a lesson from bone morphogenetic protein 8. Oncotarget. Editorial 7;8(49): 84641-84642. doi: 10.18632/ oncotarget.21597.
- Wu FJ, Lin TY, Sung LY, Chang WF, Wu PC, . (2017) BMP8A sustains spermatogenesis by activating both SMAD1/5/8 and SMAD2/3 in spermatogonia. Science Signaling. 10 (477). pii: eaal1910. doi: 10.1126/scisignal.aal1910.
- Huang WL, Li Z, Lin TY, Wang SW, Wu FJ, . (2016) Thyrostimulin-TSHR signaling promotes the proliferation of NIH:OVCAR-3 ovarian cancer cells via trans-regulation of the EGFR pathway. Scientific Reports. 6:27471. doi: 10.1038/srep27471 (PubMed).
- Lin TY, Wu FJ, Chang CL, Li Z, *. (2016) NMU signaling promotes endometrial cancer cell progression by modulating adhesion signaling. Oncotarget. 3. doi: 10.18632/oncotarget.7169. (PubMed).
- Lin TY, Huang WL, Lee WY, *. (2015) Identifying a Neuromedin U Receptor 2 Splice Variant and Determining Its Roles in the Regulation of Signaling and Tumorigenesis In Vitro. PLoS One.10(8):e0136836. (PubMed).
- Hsu PJ, Wu FJ, Kudo M, Hsiao CL, Hsueh AJ, *. (2014) A naturally occurring lgr4 splice variant encodes a soluble antagonist useful for demonstrating the gonadal roles of lgr4 in mammals. PLoS One, 9(9),e106804. doi: 10.1371/journal.pone.0106804. (PubMed).
- Deng C, Reddy P, Cheng Y, , Hsiao CL, Hsueh AJ*. (2013) Multi-functional norrin is a ligand for the LGR4 receptor. Journal of Cell Science, 126, 2060-2068. (PubMed).
- Lin TY, Wu FJ, Lee WY, Hsiao CL, * (2013) Ovarian regulation of neuromedin U and its local actions in the ovary, mediated through neuromedin U receptor 2. Am J Physiol Endocrinol Metab., 304, E800-9. (PubMed).
- Lee WY, Wang CJ, Lin TY, Hsiao CL, * (2013) CXCL17, an orphan chemokine, acts as a novel angiogenic and anti-inflammatory factor. Am J Physiol Endocrinol Metab., 304, E32-40. (PubMed).
- Hung WT, Wu FJ, Wang CJ, * (2012) DAN (NBL1) Specifically Antagonizes BMP2 and BMP4 and Modulates the Actions of GDF9, BMP2, and BMP4 in the Rat Ovary. Biology of Reproduction. 86, 1-9. (PubMed).
- Tseng HC, Tang JB, Sudhakar Gandhi PS, , Ou CM, Tseng CJ, Lin HJ*, Chen YH*. (2012) Exclusive expression of a membrane-bound Spink3-interacting serine protease-like protein TESPL in mouse testis. Amino Acids. 42, 951-960. (PubMed).
- Ou CM, Lin SR, Lin HJ, *, Chen YH.* (2010) Exclusive expression of a membrane-bound Spink3-interacting serine protease-like protein TESPL in mouse testis. J Cell Biochem. 110, 620-629. (PubMed).
- Sun SC, Hsu PJ, Wu FJ, Li SH, Lu CH, * (2010) Thyrostimulin, but not thyroid-stimulating hormone, acts as a paracrine regulator to activate thyroid-stimulating hormone receptor in the mammalian ovary. J Biol Chem. 285, 3758–3765 (PubMed)- selected for Faculty of 1000 Medicine.
- Wang CJ, Hsu SH, Hung WT, * (2009) Establishment of a chimeric reporting system for the universal detection and high-throughput screening of G protein-coupled receptors. Biosensors and Bioelectronics. 24, 2298-2304. (PubMed).
- Cho CC, , Hsu CH. (2008) Crystallization and preliminary X-ray diffraction analysis of PAT, an acetyltransferase from Sulfolobus solfataricus. Acta Crystallogr Sect F Struct Biol Cryst Commun. F64, 1049–1051. (PubMed).
- Zhang JV, Jahr H, , Klein C, Van Kolen K, Ver Donck L, De A, Baart E, Li J, Moechars D, Hsueh AJ. (2008) Obestatin induction of early response gene expression in gastrointestinal and adipose tissues and the mediatory role of GPR39. Mol Endocrinol. 22, 1464-1475. (PubMed).
- Dai L, Dewey EM, Zitnan D, , Honegger HW, Adams ME. (2008) Identification, developmental expression, and functions of bursicon in the tobacco hawkmoth, Manduca sexta. Journal of comparative neurology. 506, 759-774. (PubMed).
- Hsu SH, * (2007) Molecular Dissection of G protein Preference Using G{alpha}s Chimeras Reveals Novel Ligand Signaling of GPCRs. Am J Physiol Endocrinol Metab. 293, E1021-9. (PubMed).
- Lin HJ, , Wang CY, Chen YH. (2006) Epitope topology and removal of mouse acrosomal plasma membrane by P12-targeted immunoaggregation. Biochemical and biophysical research communications. 349, 284-288. (PubMed).
- , Hsueh AJ. (2006) Genomic analyses of the evolution of LGR genes. Chang Gung Medical Journal. 29, 2-8. (PubMed).
- Zhang JV, Ren PG, Avsian-Kretchmer O, , Rauch R, Klein C, Hsueh AJ. (2005) Obestatin, a peptide encoded by the ghrelin gene, opposes ghrelin's effects on food intake. Science. 310, 996-999. (PubMed).
- Mazerbourg S, Sangkuhl K, , Sudo S, Klein C, Hsueh AJ. (2005) Identification of receptors and signaling pathways for orphan bone morphogenetic protein/growth differentiation factor ligands based on genomic analyses. J Biol Chem. 280, 32122-32132. (PubMed).
- , Dewey EM, Sudo S, Ewer J, Hsu SY, Honegger HW, Hsueh AJ. (2005) Bursicon, the insect cuticle-hardening hormone, is a heterodimeric cystine knot protein that activates G protein-coupled receptor LGR2. Proc Natl Acad Sci U S A. 102, 2820-2825. (PubMed).
- , Pisarska MD, Hsueh AJ. (2005) Identification of a stanniocalcin paralog, STC2, in fish and the paracrine actions of STC2 in the mammalian ovary. Endocrinology. 146, 469-476. (PubMed).
- Lin HJ, Lee CM, , Chen YH. (2005) Functional Preservation of duplicated pair for RSVS III gene in the REST locus of rat 3q42. Biochem Biophys Res. Commun. 326, 355-363. NSC92-2311-B-002-019 and NSC92-2311-B-001-089. (PubMed).
- Morita H, Mazerbourg S, Bouley DM, , Kawamura K, Yoshimitsu K, Baribault H, Tian H, Hsueh AJ. (2004) Neonatal lethality of LGR5 null mice is associated with ankyloglossia and gastrointestinal distension. Mol Cell Biol. 22, 9736-9743. (PubMed).
- , Kawamura K, Klein C, Hsueh AJ. (2004) Paracrine regulation of ovarian granulosa cell differentiation by stanniocalcin 1 (STC1): mediation through specific STC1 receptors. Mol Endocrinol. 18, 2085-2096. (PubMed).
- , Lin HJ, Gopinath SC, Chen YH. (2004) Distinction of sperm-binding site and reactive site for trypsin inhibition on p12 secreted from the accessory sex glands of male mice. Biol Reprod. 70, 965-971. NSC91-2311-B001-076/NSC91-2311-B002-049. (PubMed).